白秀娟，侯 刚，卢伙胜，冯 波
（广东海洋大学水产学院，广东 湛江 524088）

摘要：为研究不同温度条件下文昌鱼对铜离子（Cu2+）和锌离子（Zn2+）的耐受性。通过净水急性毒性试验，获得了12℃、18℃、24℃、30℃和36℃条件下水中Cu2+和Zn2+对文昌鱼的24 h半致死浓度（24 h LC50）。Cu2+的24 h LC50分别为0.88,0.95,0.9,0.8,0.68 mg/L；Zn2+为4.26,4.26,4,3.8,3.67 mg/L。通过比较不同温度条件下的24 h LC50，找到文昌鱼比较适合生活的水温为18℃；相同温度条件下，Cu2+对文昌鱼的24 h LC50远低于Zn2+对文昌鱼的24 h LC50，说明Cu2+对文昌鱼的毒性远大于Zn2+对文昌鱼的毒性。在试验水温范围内，超过18℃，水温越高，文昌鱼出砂率越高，存活率降低；Cu2+和Zn2+浓度越高，文昌鱼出砂率越高，存活率越低。

关键词：文昌鱼；Cu2+；Zn2+；耐受性；温度

Study on tolerance of the Branchiostoma belcheri to Cu2+ and Zn2+ under different temperature
(Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China)
BAI Xiujuan，HOU Gang，LU Huosheng，FENG Bo

Abstract: To study the tolerance of the Branchiostoma belcheri in the Cu2+ and Zn2+ water under different temperature.water. Through the acute toxicity testing ,we found the 24h median lethal concentration(24h LC50) of the Branchiostoma belcheri in the Cu2+ water under the temperature of 12℃、18℃、24℃、30℃ and 36℃.It was 0.88mg/L、0.95 mg/L、0.9 mg/L、0.8 mg/L and 0.68 mg/L. And the 24h LC50 of the Branchiostoma belcheri in the Zn2+ water under the temperature of 12℃、18℃、24℃、30℃ and 36℃ was 4.26 mg/L、4.26 mg/L、4 mg/L、3.8 mg/L and 3.67 mg/L respectively. To compare of the 24h LC50 under different temperature, to find the most suitable temperature was 18℃ for the Branchiostoma belcheri. The 24h LC50 of Branchiostoma belcheri in the Cu2+ water far less than the 24h LC50 of Branchiostoma belcheri in the Zn2+ water. The 24h LC50 smaller values greater toxicity. We could infer the oxicity of the Cu2+ more than the Zn2+. When the temperature higher than 18℃.The Branchiostoma belcheri got out from the sand more and more and the survival rate lower when the temperature higher and higher. The concentration of Cu2+ and Zn2+ is higher the survival rate of the Branchiostoma belcheri is lower and the Branchiostoma belcheri got out from the sand more and more.

Key words: Branchiostoma belcheri; Cu2+；Zn2+; tolerance; temperature

（来源：《渔业现代化》2013,40(5):14-17）