刘伟娜1，吴 垠1，徐 哲2，孙建明2
（1 大连水产学院生命科学与技术学院，大连，116023；2 大连汇新钛设备开发有限公司，大连，116030）
摘要：为了解决在光生物反应器养殖微藻过程中，溶解氧和pH值二个培养工艺参数的控制问题，分别在80 L、350 L和900 L三种规格的气升式光生物反应器中培养湛江等鞭金藻（1sochrysis zhanjiangensis），高藻细胞浓度分别达到900×104、700×104和500×104 cell/mL，测定其中溶氧和pH值的日变化。结果显示，在光照度4 000 lx以上时，湛江等鞭金藻光合作用强，表现为反应器中藻液溶氧较高，日最高溶氧分别可达17.91 mg/L、15.84 mg/L和12.7 mg/L。所测定的藻液日pH值均在7.0～9.16内变化。
关键词：光生物反应器；湛江等鞭金藻；pH值；溶解氧；微藻细胞浓度
The change of dissolved oxygen and pH in the marine microalgae cultivation of
air-life photobioreactor
LIU Wei-na1, WU Yin1, XU Zhe2, SUN Jian-ming2
(1 College of Life Science and Biotechnology, Dalian Fishery University, Dalian 116023, China;
2 Dalian Huixin Tianium Equipment Development Co., Dalian 116039, China)
Abstract: To provides reference frame for highly cultivation of microalgae in the air-lift photobioreator. The changes of dissolved oxygen (DO) and pH on the 900×104 cell/mL, 700×104 cell/mL, 500×104 cell/mL 1sochrysis zhanjiangensis cultivation in 80 L, 350 L, 900 L photobioreators were investigated. The photosynthesis intensity of 1sochrysis zhanjiangensis was high when the light strength was above 4 000 lx. Under three densities, 900×104 cell/mL, 700×104 cell/mL, 500×104 cell/mL, the highest DO could reach 17.91 mg/L,15.84 mg/L,12.7 mg/L separately; Compared with 80 L seed-size photobioreator, 350 L and 900 L high capability photobioreators were also good for the growth of 1sochrysis zhanjiangensis. The variety of pH values in each photobioreators were in 7.0~9.16 scopes.
Key words: photobioreactor; 1sochrysis zhanjiangensis; pH; dissolved oxygen; microalgae density
作者简介：刘伟娜（1983-），女，硕士研究生。E-mail：wnliu0506@hotmail.com
通讯作者：孙建明（1960-），教授级高级工程师，主要从事水产动物工艺学及设备研究。Email: sjm@dlhuixin.com
（《渔业现代化》 2008，35（2），6-10）
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