蔡恒江1，2，李 博1，刘长发1，2
（1 大连海洋大学海洋科技与环境学院，辽宁 大连 116023；
2 辽宁省高校近岸海洋环境科学与技术重点实验室，辽宁 大连 116023）

摘要：通过实验生态学和生物化学的方法，研究了赤潮异弯藻[Heterosigma akashiwo（Hada）Hada]对孔石莼（Ulva pertusa Kjellman）生长及其叶绿素a（Chl-a）含量、丙二醛（MDA）含量、总抗氧化能力（T-AOC）、过氧化氢酶（CAT）活力、超氧化物歧化酶（SOD）活力和谷胱甘肽过氧化酶（GPX）活力的影响。结果表明：孔石莼的克生作用会明显抑制赤潮异弯藻的生长，同时赤潮异弯藻也会抑制孔石莼的生长，且孔石莼生理生化特性对其有明显的响应。赤潮异弯藻会使孔石莼CAT活力和MDA含量呈现升高的趋势；GPX活力呈现下降的趋势；T-AOC和T-SOD活力呈现先下降后升高趋势；Chl-a含量并无明显变化。推测赤潮异弯藻克生作用诱导孔石莼产生活性氧自由基，是藻体受损伤的主要原因。
关键词：赤潮异弯藻；孔石莼；生理特征；活性氧自由基

Effects of Heterosigma akashiwo (Hada) Hada on the growth and physiological characteristics of Ulva pertusa Kjellm
CAI Heng-jiang1, 2，LI Bo1，LIU Chang-fa1, 2
( 1 College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China;
2 Key Laboratory of Nearshore Marine Environmental Research, Dalian 116023, China )

Abstract: The effects of Heterosigma akashiwo（Hada）Hada on the growth, physiological characteristics including Chl-a content, MDA content, T-AOC, CAT activity, SOD activity and GPX activity of Ulva pertusa Kjellman were studied by experimental ecology and biochemical methods. The results demonstrated that U. pertusa could inhibit the growth of H. akashiwo, and H. akashiwo could inhibit the growth of U. pertusa simultaneously. The effects of U. pertusa on physiological characteristics were obvious. The MDA content and CAT activity showed increasing trend, but GPX activity showed decreasing trend while T-AOC and T-SOD showed decreasing trend from 3 d to 6 d and increasing trend from 9 d to 12 d under allelopathy from H. akashiwo. The change of Chl-a content was not obvious. Superfluous reactive oxygen species produced by allelopathy from H. akashiwo was main reasons for the damage of U. pertusa.
Key words: Ulva pertusa Kjellm; Heterosigma akashiwo (Hada) Hada; physiological characteristics; reactive oxygen species (ROS)

基金项目：海洋公益性行业科研专项（200805069）；国家自然科学基金（41171389）；辽宁省博士启动基金（20081076）；辽宁省教育厅高校科研项目（2009A174）
作者简介：蔡恒江(1978—)，男，副教授，博士，主要从事海洋生态学研究。E-mail:caihj@dlou.edu.cn

（来源：《渔业现代化》2012,39(2):16-20）