周 游^1,2，黄 滨²，宋协法¹，翟介明³，高淳仁²，梁 友²，王 峰⁴
（1中国海洋大学水产学院，山东 青岛 266003；2 中国水产科学研究院黄海水产研究所，山东 青岛 266071；
3 山东莱州明波水产有限公司，山东 烟台264000；4 青岛农业大学，山东 青岛 266109）

摘要：为了进一步优化封闭式循环水处理的系统工艺和运行参数，通过循环水养殖半滑舌鳎(cynoglossus semilaevis)的试验手段，将循环水处理系统工艺中的臭氧投加位置进行前置与后置的比较分析，探讨臭氧工艺变化对半滑舌鳎循环水养殖系统水环境的影响。结果显示，养鱼池进水口化学需氧量（COD）浓度都随着氧化还原电位（ORP）的增加而降低，臭氧后置比前置COD浓度下降更快，在ORP达到356 mv时， COD浓度降低29.38%；养鱼池进水口的氨氮、亚硝酸氮浓度后置低于前置；随着臭氧添加浓度的增加，系统对COD、氨氮、亚硝酸氮的去除率都显著增加（P<0.05），且当达到356 mv时，后置时系统对COD、氨氮、亚硝酸氮的去除率达到最大分别为34.89%、50.63%、20.64%。结果表明臭氧最佳的投加位置在循环水处理工艺的后端，臭氧投加量控制在ORP指标350 mv时，对氨氮、亚硝酸氮的去除效果更具优势，并可清新水质，节省纯氧用量。

关键词：半滑舌鳎，臭氧，封闭循环水，氧化还原电位

Effect of ozone dosing location changed on water quality in enclosed recirculating aquaculture system for tongue sole ( cynoglossus semilaevis )
ZHOU You1,2，HUANG Bin1，SONG Xie-fa2，ZHAI Jie-ming3，GAO Chun-ren1，LIANG You1，WANG Feng4

( 1 Key Laboratory for Sustainable Utilization of Marine Fisheries Resources，Ministry of Agriculture,
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Fishery College, Ocean University of China, Qingdao 266003, China;
3 LaiZhou MingBo Aquatic CO., LTD, Yantai 264000, China;
4 Qingdao agricultural university,Qingdao 266003, China)

Abstract: In order to further optimize system process and operation parameters in enclosed recirculating aquaculture system, using cynoglossus semilaevis breeding test method, Change on ozone dosing location in front of process and rear end，then comparison of the system cultivation water environmental variation situation. The paper studies that effect of ozone dosing location changed on water quality in enclosed recirculating aquaculture system for tongue sole. The results show that COD concentration in inlet of tank decreases with the increase in ORP, the COD concentration in rear decreases faster than the lead ,when ORP control in 356mv，COD concentration decreases 29.38%；The ammonia nitrogen, nitrite nitrogen in rear are lower than lead in inlet of tank；And with the increasing of the concentration of ozone add, the system on the COD and ammonia nitrogen, nitrite nitrogen removal rate are significantly increased (P <0.05)，when ORP control in 356mv, Rear of COD, ammonia nitrogen, nitrite nitrogen removal rate reached 34.89%, 50.63%, 20.64%. The results show that the best ozone dosing position is in the end of process, when ORP control in 350mv, the ammonia nitrogen，nitrite nitrogen removal effection are more advantages, and pure and fresh water quality, save pure oxygen consumption.

Key words: cynoglossus semilaevis; ozone; closed recirculation aquaculture system; oxidation reduction potential

基金项目：公益性行业（农业）科研专项（201003024）；国家鲆鲽类产业技术体系（CARS-50-G10）
作者简介：周游（1987—）男，硕士研究生，研究方向：工厂化循环水养殖系统。E-mail: zhouyou.tianxia@163.com
通讯作者：黄滨（1962—），男，副研究员，主要从事设施渔业研究。E-mail: huangbin@ysfri.ac.cn

（来源：《渔业现代化》2012,39(6):6-9,55）