刘 倩1,2，徐桂梅1，罗国芝1
（1 上海海洋大学水产与生命学院，上海市水产养殖工程技术研究中心，上海 201306；
2 济南大学泉城学院，山东 蓬莱 265600)

摘要：为考察水力停留时间（HRT）对不同硝酸盐（NO3--N）浓度的养殖污水脱氮效果的影响，建立以聚己内酯（PCL）为碳源和生物膜载体的固相反硝化反应器，经历20 d培养反应器成功启动。试验结果表明，当进水NO3--N浓度分别为100 mg/L以下、150 mg/L、200~300 mg/L时，反应器的最佳HRT分别为4、5.5和6 h，出水NO3--N浓度达到最低值，分别为17.9 mg/L、23.9 mg/L和34.1~47.4 mg/L，同时溶解性有机碳（DOC）没有大幅增加。反应器对氨氮（NH4+-N）亦有一定的去除效果，在反应器启动运行后，出水氨氮（NH4+-N）浓度明显下降，且在不同进水NO3--N及HRT下均稳定在5 mg/L左右。出水亚硝酸盐氮（NO2--N）一直维持在0.14 mg/L以下。反应器对养殖污水中的溶氧（DO）和pH变化有一定抗性，缓冲能力较强。本研究对水产养殖脱氮的实验室研究和实际运行、管理具有参考意义。

关键词：水力停留时间；硝酸盐氮负荷；反硝化；聚己内酯

Study on the effect of hydraulic retention time on aquaculture wastewater treatment in solid-phase denitrification system
LIU Qian1,2，XU Guimei1，LUO Guozhi1
(1College of Fisheries and Life Science, Shanghai Ocean University, Shanghai Aquaculture Engineering and Technology Research Center, Shanghai 201306, China;
2 Quancheng College, University of Jinan Penglai 265600, China)

Abstract: The purpose of this paper is to study the effect of hydraulic retention time (HRT) on the nitrogen removal efficiency of aquaculture wastewaters with different influent nitrate nitrogen (NO3--N) concentration. The solid-phase denitrification reactor was established by polycaprolactone (PCL) used as carbon source and biofilm carrier, which was successfully started after a period of 20 days cultivation. The results showed that, when the influent concentration of nitrate nitrogen (NO3--N) was less than 100 mg / L, 150mg / L, 200-300mg / L respectively, the best HRT of the reactor was relatively 4h, 5.5 h, 6 h. The effluent nitrate concentrations would reach to the lowest value, that was 17.9mg/L, 23.9mg/L, 34.1-47.4mg/L respectively and dissolved organic carbon (DOC) would not greatly increase. There was also a certain removal effect for Ammonia nitrogen (NH4+-N), after the start of the reactor, the effluent NH4+-N concentration decreased significantly, and was stable to around 5mg/L even under different influent nitrate concentrations and HRTs. Effluent nitrite nitrogen (NO2--N) concentration maintained at below 0.14 mg/L. There was a certain resistance for the reactor to DO and pH changes in the aquaculture wastewater, which indicted that it had a strong buffer capacity. The results of this study provided an important reference for lab experiment and study, operation, management for the nitrogen removal of aquaculturewasterwater.

Keywords: hydraulic retention time; nitrate nitrogen load; denitrification; polycaprolactone